Common name
|
B-Tg(mAbcb1b) MC38
|
Catalog number
|
322144
|
Aliases
|
md; Pgy; mdr; Abcb; Mdr1;
Pgy-; Pgy1; Abcb1; Mdr1b; Pgy-1
|
Disease
|
Colon carcinoma
|
Organism
|
Mouse
|
Strain
|
C57BL/6
|
Tissue types
|
Colon
|
Tissue
|
Colon
|
Description
The exogenous promoter and mouse
Abcb1b coding sequence were inserted into the mouse genome randomly. Mouse Abcb1b is highly expressed on the surface of B-Tg(mAbcb1b) MC38 cells.
Application
B-Tg(mAbcb1b) MC38 cells have the capability to establish tumors
in vivo and can be used for efficacy studies.
Targeting strategy
Gene targeting strategy for B-Tg(mAbcb1b) MC38 cells. The exogenous promoter and mouse
Abcb1b coding sequence were inserted into the mouse genome randomly.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-Tg(mAbcb1b) MC38 cells. B-Tg(mAbcb1b) MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-Tg(mAbcb1b) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.