Common name
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B-Tg(mCd70) MC38
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Catalog number
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321905
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Aliases
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Tnfs; Cd27l; CD27LG; Tnfsf7; Tnlg8a
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Disease
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Colon carcinoma
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Organism
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Mouse
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Strain
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C57BL/6
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Tissue types
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Colon
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Tissue
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Colon
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Description
The exogenous promoter and mouse
Cd70 coding sequence were inserted into the mouse genome randomly. Mouse
Cd70 is highly expressed on the surface of B-Tg(mCd70) MC38 cells.
Application
B-Tg(mCd70) MC38 cells have the capability to establish tumors
in vivo and can be used for efficacy studies.
Targeting strategy
Gene targeting strategy for B-Tg(mCd70) MC38 cells. The exogenous promoter and mouse
Cd70 coding sequence were inserted into the mouse genome randomly.
Protein expression analysis
CD70 expression analysis in B-Tg(mCd70) MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-Tg(mCd70) MC38 cultures were stained with species-specific anti-CD70 antibody. Mouse CD70 was detected on the surface of B-Tg(mCd70) MC38 cells but not wild-type MC38 cells. The 1-E08 clone of B-Tg(mCd70) MC38 cells was used for in vivo experiments.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-Tg(mCd70) MC38 cells. B-Tg(mCd70) MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 9-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-Tg(mCd70) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
Cell Line Inoculation Precautions:
The cell inoculation amount is between 5E5-1E6.
Tumor growth curve of individual mice
B-Tg(mCd70) MC38 tumor cells growth of individual mice. B-Tg(mCd70) MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 9-week-old, n=6). As shown in panel, B-Tg(mCd70) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.