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B-hC5AR1 mice
Strain Name C57BL/6-C5ar1tm1(C5AR1)/Bcgen Common Name  B-hC5AR1 mice
Background C57BL/6 Catalog number  110078
Aliases 
C5A; C5AR; C5R1; CD88

Gene Description


The receptor for C5a plays a critical role in numerous inflammatory conditions. C5a is a member of the complement cascade, an important mechanism for host defense against bacteria. Increased complement activation and excessive production of C5a have been implicated in the pathogenesis of several inflammatory diseases, and considerable effort has gone into developing C5aR antagonists, including organic small molecules and peptide antagonists. As one of the most potent mediators of inflammation, the complement component C5a is a promising target for anti-inflammatory drugs. However, a drawback of using animal models to develop antagonists of complement activation is that interspecies differences in receptors and ligands may complicate drug development and delay the evaluation of their efficacy in humans.  We reasoned that the availability of human C5aR knock-in mice might provide an effective means to validate this receptor in mouse models of disease, using reagents that could be developed for use in clinical trials.


Protein expression analysis(Bone marrow)

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Strain specific C5AR1 expression analysis in homozygous B-hC5AR1 mice by flow cytometry. Bone marrow were collected from WT and homozygous B-hC5AR1 (H/H) mice, and analyzed by flow cytometry with species-specific anti-C5AR1 antibody. Mouse C5AR1 was detectable in WT mice. Human C5AR1 was exclusively detectable in homozygous B-hC5AR1 dendritic cells but not WT mice. 


Protein expression analysis(MC38 cell line & MC38 intratumora)


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Strain specific C5AR1 expression analysis in MC38 cell line and MC38 intratumora by flow cytometry. MC38 cells were subcutaneously inoculated into C57BL/6 mice, and tumors were removed and prepare tumor cells when tumor volume reached appropriate size. MC38 cells and MC38 intratumora cells were collected and analyzed by flow cytometry with species-specific anti-C5AR1 antibody. Mouse C5AR1 was detectable in intratumora CD45+ cells but not in MC38 cells and intratumora CD45- cells.


Protein expression analysis(MC38 cell line & MC38 intratumora)



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Strain specific C5a expression analysis in MC38 cell culture supernatant and MC38 tumor tissue grinding solution by flow ELISA. MC38 cells were subcutaneously inoculated into C57BL/6 mice, tumors were removed and prepare when tumor volume reached appropriate size. MC38 cell culture supernatant and MC38 tumor tissue grinding solution were collected and analyzed by ELISA with mice complement C5a ELISA kit. Mouse C5A was detectable in tumor tissus suspension but not in MC38 cell culture supernatant.


Protein expression analysis(Pan02 cell line & Pan02 intratumora)


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Strain specific C5AR1 expression analysis in Pan02 intratumora by flow cytometry. Pan02 cells were subcutaneously inoculated into C57BL/6 mice, and tumors were removed and prepare tumor cells when tumor volume reached appropriate size. Pan02 intratumora cells were collected and analyzed by flow cytometry with species-specific anti-C5AR1 antibody. Mouse C5AR1 was detectable in intratumora mCD11b+mGr-1+ cells and mCD11b+mGr-1+mLy-6G+ cells.


Protein expression analysis(Pan02 cell line & Pan02 intratumora)



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Strain specific C5a expression analysis in Pan02 cells, MC38 cells culture supernatant and Pan02, MC38 tumor tissue grinding solution by ELISA. Pan02 and MC38 cells were subcutaneously inoculated into C57BL/6 mice, tumors were removed and prepare when tumor volume reached appropriate size. Pan02 cells, MC38 cells culture supernatant and Pan02, MC38 tumor tissue grinding solution were collected and analyzed by ELISA with mice complement C5a ELISA kit. Mouse C5A was detectable in tumor tissus suspension but not in cell culture supernatant.