Description

• S1PR4 (Sphingosine-1-phosphate receptor 4) is a member of the G protein-coupled receptor (GPCR) family. It primarily binds to sphingosine-1-phosphate (S1P) and is involved in regulating various physiological and pathological processes. It is predominantly expressed in the immune system.
• The exon 1 of human S1PR4 gene that encodes the full-length protein and 3’UTR was inserted into the exon 1 of mouse S1pr4 in B-hS1PR4 mice. The promoter and 5’UTR region of mouse gene are retained. The human S1PR4 expression is driven by endogenous mouse S1pr4 promoter.
• Human S1PR4 mRNA  was exclusively detectable in homozygous B-hS1PR4 mice.
• S1PR4 protein was detected in spleen and thymus of wild-type C57BL/6N mice and homozygous B-hS1PR4 mice, as the antibody can cross-recognize both human and mouse S1PR4.
• Application: This product is used for pharmacodynamics and safety evaluation of  drugs.

Targeting strategy

Gene targeting strategy for B-hS1PR4 mice. The exon 1 of human S1PR4 gene that encodes the full-length protein and 3’UTR was inserted into the exon 1 of mouse S1pr4 in B-hS1PR4 mice. The promoter and 5’UTR region of mouse gene are retained. The human S1PR4 expression is driven by endogenous mouse S1pr4 promoter.

mRNA expression analysis

Species specific analysis of S1PR4 gene expression in wild-type C57BL/6N mice and homozygous humanized B-hS1PR4 mice by RT-PCR. Spleen was collected from wild-type C57BL/6N mice (+/+) and homozygous B-hS1PR4 mice (H/H). Mouse S1pr4 mRNA was only detectable in wild-type C57BL/6N mice. Human S1PR4 mRNA was exclusively detectable in homozygous B-hS1PR4 mice but not in wild-type C57BL/6N mice.

Protein expression analysis

Western blot analysis of S1RP4 protein expression in homozygous B-hS1RP4 mice. Various tissue lysates were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hS1RP4 mice (H/H), and then analyzed by western blot with cross reactive anti-S1RP4 antibody (Invitrogen, PA5-102054). 40 μg total proteins were loaded for western blotting analysis. S1RP4 was detected in spleen and thymus of wild-type C57BL/6N mice and homozygous B-hS1PR4 mice.