|Strain Name||C57BL/6-Angptl4tm1(ANGPTL4)/Bcgen||Common Name||B-hANGPTL4 mice|
||ARP4, FIAF, HARP, HFARP, NL2, PGAR, TGQTL, UNQ171, pp1158|
mRNA expression analysis
Strain specific analysis of ANGPTL4 gene expression in wild-type mice and B-hANGPTL4 mice by RT-PCR.
Mouse Angptl4 mRNA was detectable in fat of wild-type mice (+/+). Human ANGPTL4 mRNA was detectable in homozygous B-hANGPTL4 mice but not in wild-type mice.
Strain specific ANGPTL4 expression analysis in homozygous B-hANGPTL4 mice by western blot.
Fatty tissue was collected from wild-type mice (+/+) and homozygous B-hANGPTL4 mice (H/H), and analyzed by western blot. ANGPTL4 was both detectable in wild-type mice and B-hANGPTL4 mice (H/H) due to the cross-reactivity of antibody. And the levels of ANGPTL4 was higher under fasted state both in wild-type mice and B-hANGPTL4 mice.