|Strain Name||C57BL/6-Ccl2tm1(CCL2)/Bcgen||Common Name||B-hCCL2 mice|
HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
Gene targeting strategy for B-hCCL2 mice. The full coding region sequence of mouse Ccl2 was replacement by counterpart human CCL2 sequences in B-hCCL2 mice, so the mouse Ccl2 gene was depleted.
mRNA expression analysis
Strain specific analysis of CCL2 gene expression in wild-type mice and B-hCCL2 mice by RT-PCR. Mouse Ccl2 mRNA was detectable in stomach of wild-type mice (+/+). Human CCL2 mRNA was detectable only in homozygous B-hCCL2 mice (H/H) but not in wild-type C57BL/6 mice (+/+).
Protein expression analysis