| Strain Name | C57BL/6-Pcsk9tm1(PCSK9)/Bcgen | Common Name | B-hPCSK9 mice |
| Background | C57BL/6 | Catalog number | 110928 |
|
Related Genes |
proprotein convertase subtilisin/kexin type 9, FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9 | ||
|
NCBI Gene ID |
100102 | ||
mRNA expression analysis
Strain specific analysis of PCSK9 gene expression in wild-type mice and B-hPCSK9 mice by RT-PCR. Mouse Pcsk9 mRNA was detectable in liver of wild-type mice (+/+). Human PCSK9 mRNA was detectable only in homozygous B-hPCSK9 but not in wild-type mice.
Protein expression analysis
Strain specific PCSK9 expression analysis in homozygous B-hPCSK9 mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hPCSK9 mice (H/H), and analyzed by ELISA with species-specific PCSK9 ELISA kit. Mouse PCSK9 was detectable in wild-type mice. Human PCSK9 was exclusively detectable in homozygous B-hPCSK9 mice but not in wild-type mice.
Analysis of lipid metabolism after PCSK9 humanization in B-hPCSK9 mice
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