Strain Name |
BALB/cCrSlcNifdc-Cd40lgtm1(CD40LG)Bcgen/Bcgen |
Common Name |
B-hCD40L mice(C) |
Background |
BALB/cCrSlcNifdc |
Catalog Number |
113932 |
Aliases |
IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L |
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NCBI Gene ID |
959 |
Description
Targeting strategy
Gene targeting strategy for B-hCD40L mice(C). The exons 2-5 of mouse Cd40l gene that encode extracellular domain were replaced by human counterparts in B-hCD40L mice(C). The genomic region of mouse Cd40l gene that encodes transmembrane and cytoplasmic domain was retained. The promoter, 5’UTR and 3’UTR region of the mouse gene were also retained. The CD40L expression was driven by endogenous mouse Cd40l promoter, while mouse Cd40l gene transcription and translation would be disrupted.
Protein expression analysis-Thymus
Strain specific CD40L expression analysis in homozygous B-hCD40L mice(C) by flow cytometry. Thymocytes were collected from wild-type BALB/cCrSlcNifdc (+/+) and homozygous B-hCD40L mice(C) (H/Y), then stimulated by PMA and ionomycin in cell culture for 5 h with a concentration of 0.05 μg/ml and 1 μg/ml respectively, and analyzed by flow cytometry with species-specific anti-mCD40L (Biolegend, 106505) and anti-hCD40L antibody(Biolegend, 310810). Mouse CD40L was only detectable in T cells of wild-type mice after PMA/ionomycin stimulation. Human CD40L was only detectable in T cells of B-hCD40L mice(C) mice after PMA/ionomycin stimulation.