| Strain Name |
C57BL/6-Icostm7(ICOS)Icosltm1(ICOSLG)/Bcgen |
Common Name |
B-hICOS/hICOSL mice |
| Background | C57BL/6 | Catalog number | 112901 |
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Related Genes |
ICOS, AILIM, CD278, CVID1, inducible T cell costimulator; ICOSL, AU044799, B7-H2, B7RP, B7RP-1, B7h, BG071784, GI50, GL5, GL50, GL50-, GL50-B, IC, ICOS-L, Icoslg, L, LICOS, Ly115l, mKIAA0653, icos ligand |
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Targeting strategy
Gene targeting strategy for B-hICOS/hICOSL mice. The exons 1-5 of mouse Icos gene that encode the full-length protein were replaced by human ICOS exons 1-5 in B-hICOS/hICOSL mice. The exons 3-5 of mouse IcosL gene that encode the extracellular domain were replaced by human ICOSLG exons 3-5 in B-hICOS/hICOSL mice.
Protein expression analysis
Strain specific ICOS expression analysis in homozygous B-hICOS/hICOSL miceby flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hICOS/hICOSL mice (H/H; H/H) stimulated with anti-CD3ε in vivo and analyzed by flow cytometry with vopratelimab analog (in house), STIM-003 analog (in house) and GSK-3359609 analog (in house). ICOS was detectable in WT mice (+/+) and homozygous B-hICOS/hICOSL mice (H/H; H/H) due to the cross-reactivity of antibodies. Human ICOS was exclusively detectable in homozygous B-hICOS/hICOSL mice (H/H; H/H) but not in WT mice (+/+).
Protein expression analysis
Strain specific ICOSL expression analysis in homozygous B-hICOS/hICOSL mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hICOS/hICOSL mice (H/H; H/H) , and analyzed by flow cytometry with anti-human ICOSL antibody prezalumab analog (in house), antibody A and antibody B provided by the client. Human ICOSL was exclusively detectable in homozygous B-hICOS/hICOSL mice (H/H; H/H) but not in WT mice (+/+).
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