Strain Name |
BALB/cCrSlcNifdc-Igs2tm1(SIGLEC6)Bcgen/Bcgen |
Common Name |
B-hSIGLEC6 mice(C) |
Background |
BALB/cCrSlcNifdc |
Catalog Number |
113333 |
Aliases |
CD327, CD33L, CD33L1, CD33L2, CDW327, OBBP1 |
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NCBI Gene ID |
946 |
Description
Targeting strategy
Gene targeting strategy for B-hSIGLEC6 mice(C). The full coding CDS, the promoter, 5’UTR and 3’UTR sequences of human SIGLEC6 gene are inserted into mouse Hipp11 (H11) locus in B-hSIGLEC6 mice(C).
mRNA expression analysis
Species specific analysis of SIGLEC6 gene expression in wild-type BALB/c mice and homozygous humanized B-hSIGLEC6 mice(C) by RT-PCR. Spleen was collected from wild-type BALB/c mice (+/+) and homozygous B-hSIGLEC6 mice(C) (H/H). Human SIGLEC6 mRNA was detectable only in homozygous B-hSIGLEC6 mice(C), but not in wild-type BALB/c mice.
Protein expression analysis in mast cells
Strain specific SIGLEC6 expression analysis in homozygous B-hSIGLEC6 mice(C) by flow cytometry. Peritoneal lavage fluid was collected from wild-type BALB/c mice (+/+) and homozygous B-hSIGLEC6 mice(C) (H/H), and protein expression was analyzed with anti-human SIGLEC6 antibody (BD, 747915) by flow cytometry. hSIGLEC6 was only detectable in mast cells of homozygous B-hSIGLEC6 mice but not in wild-type BALB/c mice.
Protein expression analysis in T, B and NK cells
Strain specific SIGLEC6 expression analysis in homozygous B-hSIGLEC6 mice(C) by flow cytometry. Splenocytes, blood, and lymph nodes were collected from wild-type BALB/c mice (+/+) and homozygous B-hSIGLEC6 mice(C) (H/H), and protein expression was analyzed with anti-human SIGLEC6 antibody (BD, 747915) by flow cytometry. hSIGLEC6 was detectable on B and NK cells in the blood of homozygous B-hSIGLEC6 mice(C).
Protein expression analysis in neutrophils and DCs
Strain specific SIGLEC6 expression analysis in homozygous B-hSIGLEC6 mice(C) by flow cytometry. Splenocytes and blood were collected from wild-type BALB/c mice (+/+) and homozygous B-hSIGLEC6 mice(C) (H/H), and protein expression was analyzed with anti-human SIGLEC6 antibody (BD, 747915) by flow cytometry. hSIGLEC6 was only detectable on neutrophils and DCs in the blood of homozygous B-hSIGLEC6 mice( C).
Protein expression analysis in monocytes and macrophages
Strain specific SIGLEC6 expression analysis in homozygous B-hSIGLEC6 mice(C) by flow cytometry. Splenocytes and blood were collected from wild-type BALB/c mice (+/+) and homozygous B-hSIGLEC6 mice(C) (H/H), and protein expression was analyzed with anti-human SIGLEC6 antibody (BD, 747915) by flow cytometry. hSIGLEC6 was only detectable on monocytes and macrophages in blood of homozygous B-hSIGLEC6 mice(C).