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B-Tg(hLILRB1-hLILRB4) mice
Strain Name C57BL/6-TgTn(LILRB1-LILRB4)1/Bcgen Common Name   B-Tg(hLILRB1-hLILRB4) mice 
Background C57BL/6 Catalog number  110878
Related Genes 
LILRB1: ILT2, LIR1, MIR7, PIRB, CD85J, ILT-2, LIR-1, MIR-7, PIR-B
LILRB4: ILT3, LIR5, CD85K, ILT-3, LIR-5 

Gene description


Leukocyte Ig-like receptors (LIRs) are a family of immunoreceptors expressed predominantly on monocytes and B cells and at lower levels on dendritic cells and natural killer (NK) cells. All members of LIR subfamily B contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). Upon engagement the LILRBs by MHC class I or other ligands and tyrosine phosphorylation of the ITIM transduce signals by recruitment of phosphatases SHP-1, SHP-2, or SHIP, leading to negative regulation of immune cell activation. LILRBs are considered immune checkpoint factors. The receptor can also function in antigen capture and presentation. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. The important role of LILRB4 in the immune system and its differential expression in a variety of diseases make LILRB4 a potential prophylactic and therapeutic target for a variety of diseases.
LILRB1,also known as, ILT2, LIR1 and CD85j is a cell surface protein expressed on immune cells that has a known function in inhibiting the immune response. It is a member of the ILT family, which is made up of ILT1, ILT2, ILT3 and ILT4. ILT2 is most similar to ILT4. Known ligands of ILT2 include MHC-1 as well as non-classical MHC molecules such as HLA-F, HLA-G, HLA-B27 and UL18 (human CMV). The strongest known interactor of ILT2 in the human genome is HLA-G1, activate LILRB1 and transduce a suppressive signal, which inhibits the immune responses. LILRB1 is widely expressed on human immune cells, including B cells, monocytes and macrophages, dendritic cells and subsets of natural killer (NK) cells and T cells. HLA-G1 is widely expressed on the surface of various malignancies including breast, cervical, CRC, lung, gastric, pancreatic, thyroid and ovarian cancer cells as well as glioblastoma multiform, melanoma cells. Andibody binding to ILT2 inducing/enhancing an anti-tumor T-cell response, increasing T-cell proliferation, reducing cancer-induced suppressor myeloid activity, increasing natural killer cell cytotoxicity, increasing macrophage phagocytosis, increasing generation of M1 inflammatory macrophages, decreasing generation of M2 suppressor macrophages, increasing dendritic cell number in a tumor microenvironment, increasing dendritic cell activation, treating an HLA-G expressing cancer, and treating a MHC-I expressing cancer.


Protein expression analysis (F3)


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Strain specific LILRB1 expression analysis in transgenic B-Tg(hLILRB1-hLILRB4) mice by flow cytometry. T cells and B cells of blood were collected from wild-type (+/+) and transgenic B-Tg(hLILRB1-hLILRB4) mice (Tg), and analyzed by flow cytometry with species-specific anti-human LILRB4 antibody. Human LILRB1 was both detectable in T cells and B cells from B-Tg(hLILRB1-hLILRB4) mice but not wild-type mice.

Protein expression analysis (F3)

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Strain specific LILRB1 expression analysis in transgenic B-Tg(hLILRB1-hLILRB4) mice by flow cytometry. Dendritic cells and macrophages of blood were collected from wild-type (+/+) and transgenic B-Tg(hLILRB1-hLILRB4) mice (Tg), and analyzed by flow cytometry with species-specific anti-human LILRB1 antibody. Human LILRB1 was both detectable in dendritic cells and macrophages from B-Tg(hLILRB1-hLILRB4) mice but not wild-type mice.

Protein expression analysis (F3)

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Strain specific LILRB4 expression analysis in transgenic B-Tg(hLILRB1-hLILRB4) mice by flow cytometry. T cells and B cells of blood were collected from wild-type (+/+) and transgenic B-Tg(hLILRB1-hLILRB4) mice (Tg), and analyzed by flow cytometry with species-specific anti-human LILRB4 antibody. Human LILRB4 was not detectable in T cells of B-Tg(hLILRB1-hLILRB4) mice but detectable in B cells.

Protein expression analysis (F3)

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Strain specific LILRB4 expression analysis in transgenic B-Tg(hLILRB1-hLILRB4) mice by flow cytometry. Dendritic cells and macrophages of blood were collected from wild-type (+/+) and transgenic B-Tg(hLILRB1-hLILRB4) mice (Tg), and analyzed by flow cytometry with species-specific anti-human LILRB4 antibody. Human LILRB4 was both detectable in dendritic cells and macrophages from B-Tg(hLILRB1-hLILRB4) mice but not wild-type mice.