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B-hB7-H4 mice
Strain Name C57BL/6-Vtcn1tm1(VTCN1)/Bcgen Common Name  B-hB7-h4 mice
Background C57BL/6 Catalog number  110045
Related Genes 
B7-H4, B7H4, B7S1, B7X, B7h.5, PRO1291, VCTN1

Gene description


B7-H4, also known as VTCN1, is a type I transmembrane protein and member of the B7 family. Its extracellular region consists of one IgV-like and one IgC-like domain. B7-H4 expression has been reported on activated T cells, B cells, monocytes, and dendritic cells. On T cells, B7-H4 inhibits proliferation, cytokine secretion, and cytotoxicity. B7-H4 is also expressed by different carcinomas including renal, gastric, breast, ovarian and melanoma. Its expression is associated with a poor prognosis. B7-H4 protein expressed on either the cancer cell or tumor-associated macrophages (TAMs) binds the putative B7-H4 receptor on tumor associated antigen (TAA)-specific T-cells. Subsequent inhibitory signaling in the TAA-specific T cells cause cell cycle arrest, decreased proliferation, and reduced IL-2 production.

mRNA expression analysis

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Strain specific analysis of B7-H4 gene expression in wild type and B-hB7-H4 mice by RT-PCR. 
Mouse B7-h4 mRNA was detectable only in ovary of wild type mice (+/+). Human B7-H4 mRNA was detectable only in homozygous B-hB7-H4 mice (H/H) but not in wild type mice.

Protein expression analysis



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Strain specific B7-H4 expression analysis in homozygous B-hB7-H4 mice by western blot. 
Testis tissue was collected from wild type mice (+/+) and homozygous B-hB7-H4 mice (H/H) and analyzed by western blot with anti-B7-H4 antibody. Mouse B7-H4 was detectable in wild type mice. Human B7-H4 was detectable in homozygous B-hB7-H4 but not in wild type mice. Anti-B7-H4 antibody is crossly reactive with B7-H4 in human and mice.