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B-hBTLA mice
Strain Name C57BL/6-Btlatm1(BTLA)/Bcgen Common Name  B-hBTLA mice
Background C57BL/6 Catalog number   110005
Related Genes 
BTLA (B and T lymphocyte associated)

Gene description


BTLA(B and T lymphocyte associated)is a B cell and T cell attenutor, anther immune checkpoint negative regulator of the lg superfamily. It is structually similar to CTLA4 and PD-1 , and is expressed in B cells, T cells, NK cells, dendritic cells and macrophages. BTLA binds to its ligand HVEM (HVEM is a member of the  tumor necrosis factor receptor superfamily) to transmit a co-inhibition signal in the body’s anti-tumor immune response, and is associated with the immune escape mechanism of the tumor. BTLA inhibitors enhance the TCR signaling pathway, restore T cell function, and is a potential novel target for tumor biotherapy.


Protein expression analysis


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Strain specific BTLA expression analysis in homozygous B-hBTLA mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hBTLA (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-BTLA antibody. Mouse BTLA was detectable in WT mice. Human BTLA was exclusively detectable in homozygous B-hBTLA but not WT mice.


In vivo efficacy of anti-human BTLA antibodies


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Antitumor activity of anti-human BTLA antibodies in B-hBTLA mice. (A) Anti-human BTLA antibodies inhibited MC38 tumor growth in B-hBTLA mice. Murine colon cancer hHVEM MC38 cells were subcutaneously implanted into homozygous B-hBTLA mice (female, 6-7 week-old, n=5). Mice were grouped when tumor volume reached approximately 100 mm3, at which time they were treated with two anti-human BTLA antibodies with doses and schedules indicated in panel (B) Body weight changes during treatment. As shown in panel A, anti-human BTLA antibodies were efficacious in controlling tumor growth in B-hBTLA mice, demonstrating that the B-hBTLA mice provide a powerful preclinical model for in vivo evaluation of anti-human BTLA antibodies. Values are expressed as mean ± SEM.