|Strain Name||C57BL/6-Btlatm1(BTLA)/Bcgen||Common Name||B-hBTLA mice|
||BTLA (B and T lymphocyte associated)|
BTLA（B and T lymphocyte associated）is a B cell and T cell attenutor, anther immune checkpoint negative regulator of the lg superfamily. It is structually similar to CTLA4 and PD-1 , and is expressed in B cells, T cells, NK cells, dendritic cells and macrophages. BTLA binds to its ligand HVEM (HVEM is a member of the tumor necrosis factor receptor superfamily) to transmit a co-inhibition signal in the body’s anti-tumor immune response, and is associated with the immune escape mechanism of the tumor. BTLA inhibitors enhance the TCR signaling pathway, restore T cell function, and is a potential novel target for tumor biotherapy.
Protein expression analysis
Strain specific BTLA expression analysis in homozygous B-hBTLA mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hBTLA (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-BTLA antibody. Mouse BTLA was detectable in WT mice. Human BTLA was exclusively detectable in homozygous B-hBTLA but not WT mice.
In vivo efficacy of anti-human BTLA antibodies