|Strain Name||C57BL/6-Cd248tm1(CD248)/Bcgen||Common Name||B-hCD248 mice|
CD248 is a type 1 transmembrane glycoprotein found on the plasma membrane of activated mesenchymal cells. CD248 functions during embryo development and is either not expressed or found at very low levels in adult tissues. CD248 is expressed at high levels by malignant sarcoma cells, by the pericyte component of tumor vasculature and by mesenchymal cells in some fibrotic diseases. CD248 is being targeted by several experimental therapeutics including antibodies, antibody drug conjugates, as an antigen for CART cells and in therapeutic vaccines. Although the function of CD248 has yet to be fully elucidated, this protein is a potential broad scope therapeutic target.
Protein expression analysis
Lung tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD248 mice (H/H), and analyzed by western blot with anti-CD248 antibody. Mouse CD248 was detectable in wild-type C57BL/6 mice and homozygous B-hCD248 mice, as the antibody has cross-reactivity with human CD248.
mRNA expression analysis
Strain specific analysis of CD248 gene expression in wild-type C57BL/6 mice and B-hCD248 mice by RT-PCR. Mouse Cd248 mRNA was detectable in the lung of wild-type mice (+/+). Human CD248 mRNA was detectable only in homozygous B-hCD248 mice (H/H) but not in wild-type mice.