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B-hCD47 mice
Strain Name C57BL/6-Cd47tm1(CD47)/Bcgen Common Name  B-hCD47 mice
Background C57BL/6 Catalog number  110010
Aliases 
Cd47 (CD47 antigen)

Gene description


CD47 is an integrin associated protein (IAP) and is an immunoglobulin superfamily member. CD47 is widely expressed on cell surface and interacts with the signal regulatory protein α (SIRPα), thrombospondin-1 (TSP1) and integrins to mediate a series of responses including  apoptosis, proliferation and immunity. CD47 is an important “self” mark on the cell surface and inhibits macrophagocytosis by interaction with SIRPα on macrophage surface. Animal studies have shown that a CD47 antibody is an effective treatment for multiple types of tumors. CD47 is another target for tumor immunity following PD-1/PD-L1.


Protein Expression Analysis


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Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hCD47 mice were analyzed by flow cytometry. Mouse CD47+ T cells were detectable in the WT C57BL/6 mice, while human CD47+ T cells were detectable in the homozygous B-hCD47 mice.

Human CD47 Abs Toxicity Evaluation


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Homozygous B-hCD47 mice were injected with various anti-hCD47 antibodies via i.p. injection. 
Body weight was measured daily. The results indicated toxicity of different antibodies. hCD47 Ab4 and hCD47 Ab6 showed high toxicity levels, as mice died. Y-axis showed average body weight ± SEM.

Human CD47 mAb toxicity evaluation

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Homozygous B-hCD47 mice were i.p. injected with anti-hCD47 antibodies. Blood from each mice were collected and Blood Routine Test was conducted. Blood from Ab3 and Ab5 groups were not analyzed because of death of the treated mice.


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CD47 Abs toxicity evaluation


Blood biochemical analysis of toxicity in mice injected with hCD47 antibody


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Homozygous B-hCD47 mice were I.P. injected with anti-hCD47 antibodies.Blood from each mice were collected and Blood Biochemical Test was conducted. Blood from Ab3 and Ab5 groups were not analyzed because of death of the treated mice.


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Human CD47 antibody efficacy evaluation


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Modified murine colon cancer MC38 cells (mCD47 is replaced with the hCD47 extracellular domain, named as MC38-hCD47) were subcutaneously implanted into homozygous B-hCD47 mouse. Mice were divided into control and experimental groups (n=6) when the tumor size was about 150±50 mm3. Anti-hCD47 antibodies differently inhibited tumor growth in the homozygous B-hCD47 mice at 3 different doses, suggesting that B-hCD47 mice is an effective model for in vivo CD47 antibody efficacy study. The average ±SEM of tumor sizes are shown in the figure.