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B-hCD5 mice
Strain Name C57BL/6-Cd5tm1(CD5)/Bcgen  Common Name   B-hCD5 mice
Background C57BL/6 Catalog number   111125
Related Genes 
CD5, T1, LEU1

Gene description

CD5 is a cluster of differentiation, also known as T1 and Leu-1, which is expressed on the surface of thymocytes, T cells (various species) and in a subset of murine B cells known as B-1a. CD5 is a member of the SRCR family, the extracellular region of CD5 is composed of 3 type B SRCR domains (D1, D2, and D3) each one containing 8 cysteines and encoded by a single exon (in contrast to type A, which contain 6 cysteines and are encoded by two or more exons). The nature of the endogenous CD5 ligand/s (CD5L) is reported as several counter-receptors, including CD72, gp35–40, gp150, gp200, the framework region of IgVH. CD5 is initially identified as a positive modulator of TCR signaling, but the most accepted view of CD5 is that of a negative regulator of T cell receptor signaling. CD5 also plays important roles in thymocyte, mature T cell, B cell survival, Th differentiation, suggesting that CD5 mainly acts as a pro-survival molecule in B and T cells, but under some experimental or disease conditions (such as B-CLL) it might work as a proapoptotic molecule. As CD5 modulates TCR and BCR signaling, Treg generation, anergy induction, and activation induced cell death, it can be considered an immune checkpoint inhibitor that, similarly to other negative co-stimulators such as PD-1, could be used to potentiate antitumoral T cell responses or promote immunity against infections and modulate autoimmune reactions.


mRNA expression analysis


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Strain specific analysis of CD5 gene expression in WT and homozygous B-hCD5 mice by RT-PCR. Mouse Cd5 mRNA was detectable in thymocytes of wild-type (+/+) mice. Human CD5 mRNA was detectable only in H/H, but not in +/+ mice. 

Protein expression analysis

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Strain specific CD5 expression analysis in homozygous B-hCD5 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD5 (H/H) mice, and analyzed by flow cytometry with species-specific CD5 antibody. Mouse CD5 was detectable in WT mice. Human CD5 was exclusively detectable in homozygous B-hCD5 but not WT mice.