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B-hNTRK1 mice
Strain Name C57BL/6-Ntrk1tm1(NTRK1)/Bcgen Common Name  B-hNTRK1 mice
Background C57BL/6 Catalog number  110810
Aliases 
MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA

Gene description

NTRK1 encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, cognitive disability and cancer.   


mRNA expression analysis 


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Strain specific analysis of NTRK1 gene expression in wild-type C57BL/6 mice and B-hNTRK1 mice by RT-PCR. Mouse NTRK1 mRNA was detectable only in cerebellum tissue of wild-type mice (+/+). Human NTRK1 mRNA was detectable only in homozygous B-hNTRK1 mice (H/H) but not in wild-type mice. 


Protein expression analysis


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Strain specific NTRK1 expression analysis in homozygous B-hNTRK1 mice by western blotting. Cerebellum tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hNTRK1 mice (H/H) and analyzed by western blotting with anti-NTRK1 antibody. Mouse NTRK1 was detectable in wild-type mice and homozygous B-hNTRK1 mice with a known cross-reactive anti-NTRK1 antibody. The detected protein size was 140 kDa, represents a mature form of glycoprotein. Human NTRK1 was also detectable in homozygous B-hNTRK1 mice and wild-type mice with another anti-NTRK1 antibody. So this antibody maybe also a cross-reactive antibody. The detected protein size was about 110 kDa, represents a primary form of glycoprotein.