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B-hPD-L1/hCD27 mice
Strain Name C57BL/6-Cd274tm1(CD274)Cd27tm1(CD27)/Bcgen Common Name  B-hPD-L1/hCD27 mice
Background C57BL/6 Catalog number  120537
Related Genes 
CD274 (CD274 molecule)
CD27 
(CD27 molecule)

Gene description


PD-L1 (Programmed cell death ligand-1), also known as B7-H1 and CD274, is mainly expressed in antigen-presenting cells (APCs) and activated T cells, and is one of the two ligands of PD-1. The interaction between PD1 and PD-L1 plays an important role in the negative regulation of the immune response. PD-L1 is highly expressed in a variety of solid tumors. PD-1 and PD-L1 interactions can reduce T Cell Activation and promote tumor immune escape. The PD-1/PD-L1 signaling pathway can be blocked and antitumor immune response can be restored by using by anti-PD-1 or anti-PD-L1 antibodies to block the binding of PD1 to PD-L1. 

CD27 is a TNF receptor super family member. It is expressed on the surface of T and B cells, and its binding with CD70 provides a co-stimulatory signal for T and B Cell Proliferation and for B cells producing immunoglobulins. Treating mice with a CD27 agonist antibody effectively enhanced the antitumor immune response for lymphoma and B16 melanoma, providing putative targets for tumor immunotherapy. 


Targeting Strategy


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Gene targeting strategy for B-hPD-L1/hCD27 mice. 

The exon 3 of mouse Cd274 gene that encode the extracellular domain were replaced by human CD274 exon 3.
The exons 1-5  of mouse Cd27 gene that  encode the extracellular domain were replaced by human CD27 exons 1-5  in B-hPD-L1/hCD27 mice. 


Protein Expression Analysis


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Strain specific PD-L1 expression analysis in homozygous B-hPD-L1/hCD27 mice by flow cytometry. 

Splenocytes were collected from WT and homozygous B-hPD-L1/hCD27 mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-PD-L1 antibody. Mouse PD-L1 was exclusively detectable in WT mice. Human PD-L1 were exclusively detectable in B-hPD-L1/hCD27 but not WT mice.


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Strain specific CD27 expression analysis in homozygous B-hPD-L1/hCD27 mice by flow cytometry.

Splenocytes were collected from WT and homozygous B-hPD-L1/hCD27 mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-CD27 antibody. Mouse CD27 was detectable in WT and B-hPD-L1/hCD27 mice. Human CD27 were exclusively detectable in B-hPD-L1/hCD27 but not WT mice. This might result from the cross-recognition of hCD27 by anti-mCD27 antibodies.