Strain Name |
C57BL/6JNifdc-B2mtm1(B2M/HLA-B40.1/H2-D)Bcgen/Bcgen |
Common Name |
B-HLA-B40.1 mice |
Background |
C57BL/6JNifdc |
Catalog number |
113657 |
Related Genes |
IMD43; HLAA |
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NCBI Gene ID |
567, 3105 |
Description
Targeting Strategy
Gene targeting strategy for B-HLA-B40.1 mice. The B2M gene (Exon1 to Exon3) of mouse were replaced by the sequence encompassing the human B2M CDS and HLA-B*40:01 gene that included leader sequence, α1 and α2 domains ligated to a fragment of the murine H-2Db gene containing the α3, transmembrane and cytoplasmic domains.
Protein Expression Analysis in Spleen and Blood
Strain specific B2M and HLA-B40.1 expression analysis in wild-type C57BL/6JNifdc mice and homozygous humanized B-HLA-B40.1 mice by flow cytometry. Splenocytes (A) and blood (B) were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-HLA-B40.1 mice (H/H), respectively, and analyzed by flow cytometry with species-specific anti-HLA-B40.1 (Biolegend, 311406), anti-H-2Db (Biolegend, 111513), anti-hB2M (Biolegend, 395712), and anti-mB2M (BD Biosciences, 744802) antibody. HLA-B40.1 and human B2M were exclusively detectable in homozygous B-HLA-B40.1 mice.