Strain Name |
C57BL/6-Dgat2tm1(DGAT2)Bcgen/Bcgen |
Common Name |
B-hDGAT2 mice |
Background | C57BL/6 | Catalog number |
113047 |
Related Genes |
ARAT, GS1999FULL, HMFN1045 |
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NCBI Gene ID |
84649 |
Description
Targeting strategy
Gene targeting strategy for B-hDGAT2 mice. The exons 1-8 of mouse Dgat2 gene that encode the whole molecule (ATG to STOP codon), including promoter, 5’UTR and 3’UTR were replaced by human counterparts in B-hDGAT2 mice. The human DGAT2 expression is driven by endogenous human DGAT2 promoter, while mouse Dgat2 gene transcription and translation will be disrupted.
mRNA expression analysis in humanized B-hDGAT2 mice
Strain specific analysis of DGAT2 mRNA expression in wild-type C57BL/6JNifdc mice and B-hDGAT2 mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hDGAT2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human DGAT2 primers. Mouse Dgat2 mRNA was detectable in liver of wild-type C57BL/6JNifdc mice. Human DGAT2 mRNA was detectable only in liver of homozygous B-hDGAT2 mice but not in wild-type mice.
Protein expression analysis in humanized B-hDGAT2 mice
Strain specific DGAT2 expression analysis in wild-type C57BL/6JNifdc mice and humanized B-hDGAT2 mice by Western blot. Various tissue lysates were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hDGAT2 mice (H/H), and then analyzed by western blot with anti-DGAT2 antibody (Proteintech, 17100-1-AP). DGAT2 was detectable in liver and adipose of wild-type C57BL/6 mice and homozygous B-hDGAT2 mice due to the cross-reactivity of antibody.