B-hSEMA4D mice

C57BL/6-Sema4dtm1(SEMA4D)Bcgen/Bcgen • 112334

订购产品
B-hSEMA4D mice

C57BL/6-Sema4dtm1(SEMA4D)Bcgen/Bcgen • 112334

订购产品

B-hSEMA4D mice

Product nameB-hSEMA4D mice
Catalog number112334
Strain nameC57BL/6-Sema4dtm1(SEMA4D)Bcgen/Bcgen
Strain backgroundC57BL/6
NCBI gene ID10507 (Human)
AliasesA8, BB18, C9orf164, CD100, COLL4, GR3, M-sema-G, SEMAJ, coll-4
订购产品

在此页面上

  • Targeting strategy
  • Phenotypic analysis

海报

查看全部

发表文章

相关产品

查看全部
Targeting strategy

Gene targeting strategy for B-hSEMA4D mice. The chimeric SEMA4D CDS (replace the extracellular domain) was inserted in the exon 4 of mouse Sema4d gene in B-hSEMA4D mice.

Protein expression analysis (T cells)

Strain specific SEMA4D expression analysis in T cells of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.

Protein expression analysis (B cells)

Strain specific SEMA4D expression analysis in B cells of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.

Protein expression analysis (NK cells)
Strain specific SEMA4D expression analysis in NK cells of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.
Protein expression analysis (DC cells)
Strain specific SEMA4D expression analysis in DC cells of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.
Protein expression analysis (granulocytes)
Strain specific SEMA4D expression analysis in granulocytes of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.
Protein expression analysis (macrophages)
Strain specific SEMA4D expression analysis in macrophages of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.
Protein expression analysis (monocytes)

Strain specific SEMA4D expression analysis in monocytes of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.

* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hSEMA4D mice] (Cat# 112334) was purchased from Biocytogen.