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B-hTROP2 mice
Strain Name C57BL/6-Tacstd2tm1(TACSTD2)/Bcgen Common Name  B-hTROP2 mice
Background C57BL/6 Catalog number  110966
Related Genes 
TACSTD2, EGP-1, EGP1, GA733-1, GA7331, GP50, M1S1, TROP2, tumor associated calcium signal transducer 2, Trophoblast cell-surface antigen 2

Gene description


Human TROP-2, also called tumor associated calcium signal transducer 2 (TACSTD2), GA733-1, gp50 and T16, is a type I cell surface glycoprotein that is highly expressed on human carcinomas. It was originally identified as an antigen present on human gastrointestinal tumors and is the second of two members of this family. The other family member is GA733-2, also called EpCAM, TROP-1, 17-1A, gp40 and KSA. The TROP-2 gene is unique in that it contains no introns. A study of these two genes suggested that TROP-2 was the result of a retroposition of the EPCAM gene. TROP-2 and EPCAM share approximately 49% amino acid identity and approximately 67% similarity. Human and mouse TROP-2 share 87% similarity. Trop-2 mediated signaling pathways promote tumor cell growth, proliferation and metastasis mainly by regulating calcium signaling pathways, cyclin expression and reducing fibronectin adhesion.Trop-2 is highly expressed in a variety of solid tumors and has become a new target for the development of antibody-conjugated drugs. Recently, there have been many significant advances in Trop-2 antibody-conjugated drugs (ADCs), of which Sacituzumab govitecan-hziy has been marketed, and many others have entered the clinical and preclinical stages.



mRNA expression analysis


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Strain specific analysis of TROP2 gene expression in wild type (WT) mice and B-hTROP2 mice by RT-PCR. 
Mouse Trop2 mRNA was detectable only in the skin of WT mice (+/+). Human TROP2 mRNA was detectable only in homozygous B-hTROP2 mice (H/H) but not in WT mice (+/+). 

Protein expression analysis

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Strain specific TROP2 expression analysis in homozygous B-hTROP2 mice by western blot. 

Skin and kidney tissues were collected from wild type (WT) mice (+/+) and homozygous B-hTROP2 mice (H/H), and analyzed by western blot with anti-TROP2 antibody. Mouse TROP2 was detectable in WT mice (+/+) due to the cross-reactivity of antibodies. Human TROP2 was detectable in homozygous B-hTROP2 mice (H/H) and B-hTROP2 MC38 cell lines.


Analysis of spleen leukocytes cell subpopulations in B-hTROP2 mice


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Analysis of spleen leukocyte subpopulations by FACS.
Splenocytes were isolated from female C57BL/6 and B-hTROP2 mice (n=3, 6 week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45 population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hTROP2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hTROP2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in spleen. Values are expressed as mean ± SEM.

Analysis of spleen T cell subpopulations in B-hTROP2 mice

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Analysis of spleen T cell subpopulations by FACS.
Splenocytes were isolated from female C57BL/6 and B-hTROP2 mice (n=3, 6 week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3 T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of CD8+ T cells, CD4+ T cells and Treg cells in homozygous B-hTROP2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hTROP2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell sub types in spleen. Values are expressed as mean ± SEM.

Analysis of lymph node leukocytes cell subpopulations in B-hTROP2 mice

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Analysis of lymph node leukocyte subpopulations by FACS.
Leukocytes were isolated from female C57BL/6 and B-hTROP2 mice (n=3, 6 week-old). Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45 population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells and NK cells in homozygous B-hTROP2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hTROP2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in lymph node. Values are expressed as mean ± SEM.

Analysis of lymph node T cell subpopulations in B-hTROP2 mice

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Analysis of lymph node T cell subpopulations by FACS.
Leukocytes were isolated from female C57BL/6 and B-hTROP2 mice (n=3, 6 week-old). Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3 T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of CD8+ T cells, CD4+ T cells and Treg cells in homozygous B-hTROP2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hTROP2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell sub types in lymph node. Values are expressed as mean ± SEM.

Analysis of blood leukocytes cell subpopulations in B-hTROP2 mice

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Analysis of blood leukocyte subpopulations by FACS.
Blood cells were isolated from female C57BL/6 and B-hTROP2 mice (n=3, 6 week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45 population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hTROP2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hTROP2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.

Analysis of blood T cell subpopulations in B-hTROP2 mice

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Analysis of blood T cell subpopulations by FACS.
Blood cells were isolated from female C57BL/6 and B-hTROP2 mice (n=3, 6 week-old). Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3 T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of CD8+ T cells, CD4+ T cells and Treg cells in homozygous B-hTROP2 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hTROP2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell sub types in blood. Values are expressed as mean ± SEM.