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B-Igh-J KO mice
Strain Name C57BL/6-Igh-Jtm1/Bcgen  Common Name  B-Igh-J KO mice
Background C57BL/6 Catalog number  111116
Related Genes 
Igh-J (immunoglobulin heavy chain, joining region)

Gene description

Igh genetically modified mice are targeted to destroy Ig heavy chain genes, which leads to the inhibition of key stages such as B cell receptor rearrangement and splicing in this model, and the development of B cells stops in the pre-B cell stage, thus leading to the inability to produce antibodies. Igh genetically modified mice can be used as an effective screening model for IO drugs, which retain an important immune cell type for immunotherapy evaluation while not producing allergic reactions to ADAs. B cell deficient mice can effectively reduce or even avoid the production of immunogenicity, which provides more possibilities for the screening of potentially effective drugs. 

Biocytogen developed the B-Igh-J KO mice, which carries a deletion of the endogenous murine J segments of the Ig heavy chain locus. In homozygotes, all four JH gene and Ighd4-1 segments are absent, resulting in cells that cannot produce a complete, recombined version of the variable region of the heavy chain. B cell development halts at the pro-B cell stage without pre-B cell or mature B cells in the bone marrow and spleen. The B-Igh-J KO mice will be more useful for basic mechanistic studies of immunologic mechanisms as well as host mice in syngeneic tumor studies using immunogenic test articles.


Protein expression analysis


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Strain specific analysis of IgD and IgM expression in WT and the homozygous B-Igh-J KO mice by flow cytometry. Spleen were collected from WT mice and homozygous B-Igh-J KO mice (-/-). Mouse IgD and IgM were detectable in WT mice but not the homozygous B-Igh-J KO mice.

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Strain specific analysis of IgD and IgM expression in WT and the homozygous B-Igh-J KO mice by flow cytometry. Bone marrow were collected from WT mice and homozygous B-Igh-J KO mice (-/-). Mouse IgD and IgM were detectable in WT mice but not the homozygous B-Igh-J KO mice.