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B-Rag2 KO mice
Strain Name C57BL/6-Rag2tm1/Bcgen  Common Name  B-Rag2 KO mice
Background C57BL/6 Catalog number   110809
Related Genes 
Rag, Rag-2

Gene description


Rag2 encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1 (Rag1) , and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. Homozygotes for targeted null mutations exhibit arrested development of T and B cell maturation at the CD4-CD8- thymocyte or B220+CD43+ pro-B cell stage due to inability to undergo V(D)J recombination.

Biocytogen have developed the B-Rag2 KO mice, the target strategy was to delete the exon 3 and the 3’UTR region of Rag2 gene. Rag2 knock-out mice have impaired T and B-cell development and may be useful in the study of hematopoietic and immune system defects, cancer, toxicology and xenograft/transplant studies.



Analysis of immune cell subpopulations in spleen


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Analysis of splenic immune cell subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-Rag2 KO mice (n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Compared to cells in wild-type C57BL/6 mice, percent of T cells and B cells were significantly decreased, while percent of all the other cells, including NK cells, dendritic cells, granulocytes, monocytes and macrophages in B-Rag2 KO mice were  significantly increased. The results demonstrate that knockout of Rag2 gene has blocked B and T cell development and differentiation in spleen. Values are expressed as mean ± SEM.


Analysis of T cell subpopulations in spleen


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Analysis of splenic T cell subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-Rag2 KO mice (n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells and Tregs in B-Rag2 KO mice were significantly decreased compared to those in wild-type C57BL/6 mice, demonstrating that  knockout of Rag2 gene has blocked T cell development and differentiation in spleen. Values are expressed as mean ± SEM.

Analysis of immune cell subpopulations in thymus



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Analysis of thymic immune cell subpopulations by FACS. Thymuses were isolated from female C57BL/6 and B-Rag2 KO mice (n=3, 6-week-old). Flow cytometry analysis of the thymocytes was performed to assess immune cell subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Compared to the wild-type C57BL/6 mice, percent of total T cells, CD4+ T cells and CD8+ T cells were significantly decreased in B-Rag2 KO mice, while the percent of other cells including B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in B-Rag2 KO mice were similar to those in the C57BL/6 mice. Results demonstrate that knockout of Rag2 gene has blocked T cell development and differentiation in thymus. Values are expressed as mean ± SEM.

Analysis of T cell subpopulations in thymus


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Analysis of thymic T cell subpopulations by FACS. Thymuses were isolated from female C57BL/6 and B-Rag2 KO mice (n=3, 6-week-old). Flow cytometry analysis of the thymocytes was performed to assess T cell subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. CD4+ T cells, CD8+ T cells and Tregs were not detectable in homozygous B-Rag2 KO mice. Results demonstrate that knockout of Rag2 gene has completely blocked T cell development and differentiation in thymus. Values are expressed as mean ± SEM.

Analysis of immune cell subpopulations in blood

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Analysis of blood immune cell subpopulations by FACS. Blood were isolated from female C57BL/6 and B-Rag2 KO mice (n=3, 6-week-old). Flow cytometry analysis of the blood cells was performed to assess immune cell subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Compared to cells in wild-type C57BL/6 mice, percent of total T cells, CD4+ T cells, CD8+ T cells and B cells were significantly decreased, while percent of NK cells, monocytes and macrophages in B-Rag2 KO mice were significantly increased. Percent of dendritic cells and granulocytes in B-Rag2 KO mice were similar to those in wild-type C57BL/6 mice. The results demonstrate that knockout of Rag2 gene has blocked B and T cell development and differentiation in blood. Values are expressed as mean ± SEM.


Analysis of T cell subpopulations in blood


from clipboard



Analysis of blood T cell subpopulations by FACS. Blood were isolated from female C57BL/6 and B-Rag2 KO mice (n=3, 6-week-old).  Flow cytometry analysis of the blood cells was performed to assess T cell subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for TCRβ+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of CD4+ T cells, CD8+ T cells and Tregs in B-Rag2 KO mice were significantly decreased compared to those in the C57BL/6 mice, demonstrating that knockout of Rag2 gene has blocked T cell development and differentiation in blood. Values are expressed as mean ± SEM.