Strain Name |
BALB/cCrSlcNifdc-Glp1rtm1Bcgen/Bcgen |
Common Name |
B-Glp1r KO mice(C) |
Background |
BALB/cCrSlcNifdc |
Catalog number |
113758 |
Related Genes |
glucagon like peptide 1 receptor |
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NCBI Gene ID |
2740 |
Description
Gene targeting strategy for B-Glp1r KO mice(C).
Protein expression analysis:
mRNA expression analysis:
Targeting strategy
Gene targeting strategy for B-Glp1r KO mice(C). The genome sequences between exons 2-3 in the mouse Glp1r gene were depleted in B-Glp1r KO mice(C).
mRNA expression analysis-Brain
Strain specific analysis of Glp1r mRNA expression in wild-type BALB/cCrSlcNifdc mice and B-Glp1r KO mice(C) by RT-PCR. Brain RNA was isolated from wild-type BALB/cCrSlcNifdc mice and homozygous B-Glp1r KO mice(C) (-/-), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Glp1r primers. Mouse Glp1r mRNA was detectable only in wild-type BALB/cCrSlcNifdc mice but not in B-Glp1r KO mice(C).
Mouse GLP1R Protein Expression Analysis of B-Glp1r KO mice(C)
Representative mouse GLP1R expression in the pancreas tissues of B-Glp1r KO mice(C) by IHC. Tissues were stained with mouse-specific antibodies GLP1R (abcam: ab218532) (A-B). The pancreas of wild-type BALB/cCrSlcNifdc mice (+/+) showed mouse GLP1R positive (A). The pancreas of B-Glp1r KO mice(C) showed mouse GLP1R negative (B). Original magnification ×200. Abbreviations: IHC, immunohistochemistry.