Strain Name |
C57BL/6N-Mlkltm1Bcgen/Bcgen |
Common Name |
B-Mlkl KO mice |
Background |
C57BL/6N |
Catalog number |
113753 |
Related Genes |
hMLKL |
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NCBI Gene ID |
197259 |
Description
Targeting strategy
Gene targeting strategy for B-Mlkl KO mice. The exons 2-10 of mouse Mlkl gene were knockout in B-Mlkl KO mice. Mouse Mlkl gene transcription and translation will be disrupted.
Protein expression analysis
Western blot analysis of Mlkl protein expression in homozygous B-Mlkl KO mice. Various tissue lysates were collected from wild-type C57BL/6N mice (+/+) and homozygous B-Mlkl KO mice (-/-) (male, 6-week-old), and then analyzed by western blot with anti-Mlkl antibody(abcam, ab243142). 40 μg total proteins were loaded for western blotting analysis. MLKL was not detectable in liver, lung, brain, stomach and kidney of B-Mlkl KO mice.
mRNA expression analysis
Strain specific analysis of Mlkl mRNA expression in wild-type C57BL/6N and B-Mlkl KO mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6N (+/+) and homozygous B-Mlkl KO mice(-/-), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Mlkl primers. Mouse Mlkl mRNA was exclusively detectable in C57BL/6N but not detectable in B-Mlkl KO mice.