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B-Luc-EGFP HL-60
Common name

 B-Luc-EGFP HL-60

Catalog number       321821
Aliases

NA

Disease  NA
Organism
Human Strain  NA
Tissue types

Peripheral blood

Tissue  Peripheral blood

Description 


This B-Luc-EGFP HL-60 cell line expresses firefly luciferase as a marker of HL-60 cells. Fluorescence could be detected in B-Luc-EGFP HL-60 cells.


Application

B-Luc-EGFP HL-60 cell line can be used to evaluate anti-cancer drugs in acute myelogenous leukemia in vivo.

Targeting strategy

Gene targeting strategy for B-Luc-EGFP HL-60 cells. The CMV promoter and luciferase-EGFP were inserted into the targeting vector used in the lentivirus system. 

In vitro luciferase activity of B-Luc-EGFP HL-60 cells 

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Luminescence signal intensity of B-Luc-EGFP HL-60 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-Luc-EGFP HL-60 cells have a strong luminescence signal. 


Quantitative imaging data & body weight changes

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B-Luc-EGFP HL-60 cells (1x105) were injected into the tail vein of wild-type B-NDG mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc-EGFP HL-60 cells can be used for in vivo efficacy evaluation.