|Disease||Reticulum cell sarcoma|
Gene targeting strategy for B-Luc A20 cells. The exogenous promoter and luciferase was inserted into the ROSA26 locus in murine.
In vitro luciferase activity of B-Luc A20 cells
Luminescence signal intensity of B-Luc A20 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay (Promega, Cat E2610). B-Luc A20 cells have a strong luminescence signal. Clone No. :2-A10.
Quantitative imaging data & body weight changes
Tumor growth and in vivo imaging of B-Luc A20 cells. B-Luc A20 cells (5x105) were injected into the tail vein of wild-type BALB/c mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc A20 cells can be used for in vivo efficacy evaluation.
Imaging analysis in vivo