Generation of gene editing Daudi and expression analysis
Gene targeting strategy for B-luc Daudi. The luciferase cDNA sequence with CAG promoter was inserted in the AAVS1 locus exon1 of wild-type Daudi .
The luminescence signal intensity of B-luc Daudi cells. The luminescence intensity was detected by Bright-GloTM luciferase Assay System (Promega, Cat E2610). B-luc Daudi cells have strong luminescence signal.
Tumor Growth Curve & Body Weight Changes
Subcutaneous xenograft tumor growth of B-luc Daudi cells. B-luc Daudi cells and wild-type Daudi cells (5x106) were subcutaneously implanted into the B-NDG mice (n=5).Tumor size and mice body weight were measured twice a week. (A) Tumor average volume ± SEM, (B) Mice body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5a X b2, where a and b were the long and short diameters of the tumor, respectively. As shown in panel A, B-luc Daudi were able to establish tumor in vivo and can be used for efficacy study.
In vivo Imaging Analysis
A.B-luc Daudi cells were inoculated into the tail vein of B-NDG mice, and imaging was performed on days 0, 3, 7, 10, 14, 17, 21, 24, and 28 for detection and tumor imaging signal intensity analysis
B.Animal weight monitoring after B-NDG mice were inoculated with B-luc Daudi cells.
Results: B-luc Daudi cells were able to establish tumor model steadily and track tumor volume effectively .