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B-luc Daudi
Common name

 B-luc Daudi

Catalog number      310677
Aliases


Disease  Burkitt's lymphoma
Organism
Human 
Strain  Human
Tissue types

Lymphoma

Tissue

Generation of gene editing Daudi and expression analysis

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Gene targeting strategy for B-luc Daudi. The luciferase cDNA sequence with CAG promoter was inserted in the AAVS1 locus  exon1 of  wild-type Daudi .


Phenotypic Analysis

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The luminescence signal intensity of B-luc Daudi cells. The luminescence intensity was detected by Bright-GloTM luciferase Assay System (Promega, Cat E2610). B-luc Daudi cells have strong luminescence signal.


Tumor Growth Curve & Body Weight Changes

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Subcutaneous xenograft tumor growth of B-luc Daudi cells. B-luc Daudi cells and wild-type Daudi cells (5x106) were subcutaneously implanted into the B-NDG mice (n=5).Tumor size and mice body weight were measured twice a week. (A) Tumor average volume ± SEM, (B) Mice body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5a X b2, where a and b were the long and short diameters of the tumor, respectively. As shown in panel A, B-luc Daudi were able to establish tumor in vivo and can be used for efficacy study.


In vivo  Imaging Analysis


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A.B-luc Daudi cells were inoculated into the tail vein of B-NDG mice, and imaging was performed on days 0, 3, 7, 10, 14, 17, 21, 24, and 28 for detection and tumor imaging signal intensity analysis

B.Animal weight monitoring after B-NDG mice were inoculated with B-luc Daudi cells.

Results: B-luc Daudi cells were able to establish tumor model steadily and  track tumor volume effectively .