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B-luc K-562
Common name

 B-luc K-562

Catalog number      310673
Aliases 


AAVS1(adeno-associated virus integration site 1)


Disease  Leukemia (CML)
Organism

Homo sapiens

Strain  Human
Tissue types


Tissue

Generation of gene editing cell line model and expression analysis

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Gene targeting strategy for B-luc K-562. The stop-CAG-Pr-luciferase-PA- LoxP-PuroR- LoxP sequence was inserted in the hAAVS1 locus of wild-type K-562.

Phenotypic Analysis

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The luminescence signal intensity of B-luc K-562 cells. The luminescence intensity was detected by Bright-GloTM luciferase Assay System (Promega, Cat E2610), and B-luc K-562 cells have strong luminescence signal.

Imaging analysis in vivo

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Tumor growth and in vivo imaging of B-luc K-562 cells. B-luc K-562 cells (5x106) were  injected by tail vein into B-NDG mice .Signal intensity and body weight were measured twice a week. (A) Imaging was performed on days 0, 4, 7, 11, 21, and 25, (B) Mice body weight (Mean ± SEM). B-luc K-562 cells can be used for in vivo efficacy evaluation.