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B-hB7-H3 EL4
Common name
B-hB7-H3 EL4 Catalog number    310705
Aliases 4Ig-B7-H3, CD276, B7RP-2 Disease  Lymphoma
Organism
Mouse Strain  C57BL/6N
Tissue types Lymphoma Tissue  T lymphocyte

Description


The mouse B7h3 gene was replaced by human B7H3 coding sequence in B-hB7-H3 EL4 cells. Human B7-H3 is highly expressed on the surface of B-hB7-H3 EL4 cells.


Application


B-hB7-H3 EL4 cells have the capability to establish tumors in vivo and can be used for efficacy studies.


Targeting strategy


Gene targeting strategy for B-hB7-H3 EL4 cells. The exogenous promoter and human B7H3 coding sequence was inserted to replace part of murine exon 3 and all of exon 4. The insertion disrupts the endogenous murine B7h3 gene, resulting in a non-functional transcript.


Protein expression analysis




B7-H3 expression analysis in B-hB7-H3 EL4 cells by flow cytometry. Single cell suspensions from wild-type EL4 and B-hB7-H3 EL4 cultures were stained with species-specific anti-B7-H3 antibody. Human B7-H3 was detected on the surface of B-hB7-H3 EL4 cells but not wild-type EL4 cells. The 1-H02 clone of B-hB7-H3 EL4 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes




Subcutaneous homograft tumor growth of B-hB7-H3 EL4 cells. B-hB7-H3 EL4 cells (2x105) and wild-type EL4 cells (2x105) were subcutaneously implanted into C57BL/6N mice (female, 6-9-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hB7-H3 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies.Animals with tumor volume over 3000mm3 in the control group were euthanized.