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B-hC5 MC38
Common name
B-hC5 MC38 Catalog number     311324
Aliases C5Da, C5b, CPAMD4, ECLZB Disease  Colon carcinoma
Organism
Mouse
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description


The CDS of human C5 was inserted to replace part of murine exon 2 in B-hC5 MC38 cells. Human C5 is highly expressed on the surface of B-hC5 MC38 cells.

Application

B-hC5 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hC5 MC38 cells. The CDS of human C5 was inserted to replace part of murine exon 2. The insertion disrupts the endogenous murine C5 gene, resulting in a non-functional transcript.

Protein expression analysis 

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C5 expression analysis in B-hC5 MC38 cells by ELISA. Supernatant from wild-type MC38 and B-hC5 MC38 cultures were stained with species-specific anti-C5 antibody. Human C5 was detectable in the supernatant of B-hC5 MC38 cells but not in that of wild-type MC38 cells. The 1-G04 clone of B-hC5 MC38 cells was used for in vivo experiments.


Tumor growth curve & Body weight changes


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Subcutaneous homograft tumor growth of B-hC5 MC38 cells. B-hC5 MC38 cells (1x106) and wild-type MC38 cells (1x106) were subcutaneously implanted into C57BL/6N mice (female, 8-week-old, n=8). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B)  Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hC5 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.