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B-hCD19 EL4
Common name
B-hCD19 EL4 Catalog number     310712
Aliases CD19, CD19 molecule, B4, CVID3 Disease  Lymphoma
Organism
Mouse Strain  C57BL/6N
Tissue types Lymphoma Tissue  T lymphocyte

Description


The mouse Cd19 gene was replaced by human CD19 coding sequence in B-hCD19 EL4 cells. Human CD19 is highly expressed on the surface of B-hCD19 EL4 cells.


Application


B-hCD19 EL4 cells have the capability to establish tumors in vivo and can be used for efficacy studies.



Targeting strategy


Gene targeting strategy for B-hCD19 EL4 cells.

The exogenous promoter and human CD19 coding sequence was inserted to replace part of murine exons 2-12. The insertion disrupts the endogenous murine CD19 gene, resulting in a non-functional transcript.


Protein expression analysis 


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The CD19 expression analysis in B-hCD19 EL4 cells by flow cytometry. 

Single cells were collected from wild-type EL4 and B-hCD19 EL4 cells, and analyzed by flow cytometry with species-specific anti-CD19 antibody. Human CD19 was detectable in B-hCD19 EL4 cells but not wild-type EL4 cells. The 2-D07 clone of B-hCD19 EL4 cells was used for in vivo experiments.


Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hCD19 EL4 cells. 
B-hCD19 EL4 cells (2x105) and wild-type EL4 cells (2x105) were subcutaneously implanted into C57BL/6N mice (female, 6-8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean ± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD19 EL4 cells were able to establish tumors in vivo and can be used for efficacy studies. Animals with tumor volume over 3000mm3 in the control group were euthanized.