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B-hCD24 MC38
Common name
B-hCD24 MC38 Catalog number     311050
Aliases CD24,CD24A,PRO940 Disease  Colon carcinoma
Organism
Mouse
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description 

The mouse Cd24 gene was replaced by human CD24 coding sequence in B-hCD24 MC38 cells. Human CD24 is highly expressed on the surface of B-hCD24 MC38 cells.

Application


B-hCD24 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.


Targeting strategy


Gene targeting strategy for B-hCD24 MC38 cells. The exogenous promoter and human CD24 coding sequence was inserted to replace part of murine exon 1 and all of exon 2. The insertion disrupts the endogenous murine Cd24 gene, resulting in a non-functional transcript.

Protein expression analysis 

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CD24 expression analysis in B-hCD24 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCD24 MC38 cultures were stained with species-specific anti-CD24 antibody. Human CD24 was detected on the surface of B-hCD24 MC38 cells but not wild-type MC38 cells. The 1-F02 clone of B-hCD24 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hCD24 MC38 cells. B-hCD24 MC38 cells (1x106) and wild-type MC38 cells (1x106) were subcutaneously implanted into C57BL/6 mice (female, 6-7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 × long diameter × short diameter2. As shown in panel A, B-hCD24 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.