请输入关键字
请输入关键字
订购
*国家
中国
美国
*省份
*城市
*姓名
*电话
*单位
*职位
*邮箱
*请输入验证码
*验证码
B-hCD38 MC38
Common name
 B-hCD38 MC38 Catalog number     310730
Aliases  ADPRC1 Disease  Colon carcinoma
Organism
Mouse
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description 

The mouse Cd38 gene was replaced by chimeric CD38 coding sequence in B-hCD38 MC38 cells. Human CD38 is highly expressed on the surface of B-hCD38 MC38 cells.


Application


B-hCD38 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.


Targeting strategy


Gene targeting strategy for B-hCD38 MC38 cells. The exogenous promoter and chimeric CD38 coding sequence was inserted to replace part of murine exon 2 and all of exon 3. The insertion disrupts the endogenous murine Cd38 gene, resulting in a non-functional transcript.

Protein expression analysis 

from clipboard


CD38 expression analysis in B-hCD38 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCD38 MC38 cultures were stained with species-specific anti-CD38 antibody. Mouse CD38 was detected on the surface of wild-type MC38 cells. Human CD38 was detected on the surface of B-hCD38 MC38 cells but not wild-type MC38 cells. The 4-F06 clone of B-hCD38 MC38 cells was used for in vivo experiments.


Tumor growth curve & Body weight changes


from clipboard


Subcutaneous homograft tumor growth of B-hCD38 MC38 cells. B-hCD38 MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6N mice (female, 7-8-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD38 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
Recommendations for inoculation in other mouse strains: 
Cell inoculation amount is recommended to be tried between 5x105-1x107
Inoculated cells can be tried to be suspended with DMEM stock solution.