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B-hCD47 MC38
Common name
B-hCD47 MC38 Catalog number    310695
Aliases  IAP, MER6, OA3 Disease  Colon carcinoma
Genotype Homozygous Culture properties  Adherent
Organism
Mouse
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description 


The mouse Cd47 gene was replaced by human CD47 coding sequence in B-hCD47 MC38 cells. Human CD47 is highly expressed on the surface of B-hCD47 MC38 cells.

Targeting strategy

Gene targeting strategy for B-hCD47 MC38 cells. The exogenous promoter and human CD47 coding sequence was inserted to replace part of murine exon 2 and all of exon 3. The insertion disrupts the endogenous murine Cd47 gene, resulting in a non-functional transcript.


Protein expression analysis 

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CD47 expression analysis in B-hCD47 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCD47 MC38 cultures were stained with species-specific anti-CD47 antibody. Human CD47 was detected on the surface of B-hCD47 MC38 cells but not wild-type MC38 cells. The 1-B3 clone of B-hCD47 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hCD47 MC38 cells. B-hCD47 MC38 cells (1x106) were subcutaneously implanted into B-hSIRPA/hCD47 mice (female, 7-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD47 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.