||B-hCXCR2 MC38||Catalog number||310824|
|Aliases||CD182, CMKAR2, IL8R2, IL8RA, IL8RB||Disease||Colon carcinoma|
CXCR2 expression analysis in B-hCXCR2 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCXCR2 MC38 cultures were stained with species-specific anti-CXCR2 antibody. Human CXCR2 was detected on the surface of B-hCXCR2 MC38 cells but not wild-type MC38 cells. The 6-F02 clone of B-hCXCR2 MC38 cells was used for in vivo experiments.
Subcutaneous homograft tumor growth of B-hCXCR2 MC38 cells. B-hCXCR2 MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6N mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCXCR2 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.