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B-hCXCR2 MC38
Common name
B-hCXCR2 MC38 Catalog number     310824
Aliases CD182, CMKAR2, IL8R2, IL8RA, IL8RB Disease  Colon carcinoma
Organism
Mouse
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description


The mouse Cxcr2 gene was replaced by human CXCR2 coding sequence in B-hCXCR2 MC38 cells. Human CXCR2 is highly expressed on the surface of B-hCXCR2 MC38 cells.

Targeting strategy


Gene targeting strategy for B-hCXCR2 MC38 cells. The exogenous promoter and human CXCR2 coding sequence was inserted to replace part of murine exon 2 ~ 3’UTR. The insertion disrupts the endogenous murine Cxcr2 gene, resulting in a non-functional transcript.

Protein expression analysis


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CXCR2 expression analysis in B-hCXCR2 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCXCR2 MC38 cultures were stained with species-specific anti-CXCR2 antibody. Human CXCR2 was detected on the surface of B-hCXCR2 MC38 cells but not wild-type MC38 cells. The 6-F02 clone of B-hCXCR2 MC38 cells was used for in vivo experiments.


Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hCXCR2 MC38 cells. B-hCXCR2 MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6N mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCXCR2 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.