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B-hDLL3 MC38
Common name
B-hDLL3 MC38 Catalog number     311448
Aliases delta like canonical Notch ligand 3, SCDO1 Disease  Colon carcinoma
Organism
Mouse
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description 


The mouse Dll3 gene was replaced by human DLL3 coding sequence in B-hDLL3 MC38 cells. Human DLL3 is highly expressed on the surface of B-hDLL3 MC38 cells.

Application

B-hDLL3 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hDLL3 MC38 cells. 
The exogenous promoter and human DLL3 coding sequence was inserted to replace part of murine exon 2 and all of exon 5. The insertion disrupts the endogenous murine Dll3 gene, resulting in a non-functional transcript.

Protein expression analysis 

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DLL3 expression analysis in B-hDLL3 MC38 cells by flow cytometry. 
Single cell suspensions from wild-type MC38 and B-hDLL3 MC38 cultures were stained with species-specific anti-DLL3 antibody. Human DLL3 was detected on the surface of B-hDLL3 MC38 cells but not wild-type MC38 cells. The 3-G07 clone of B-hDLL3 MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes


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Subcutaneous homograft tumor growth of B-hDLL3 MC38 cells. 

B-hDLL3 MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6N mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hDLL3 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.