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B-hEPCAM MC38
Common name
B-hEPCAM MC38 Catalog number    310708
Aliases DIAR5, EGP-2, EGP40, ESA, HNPCC8, TROP1 Disease  Colon carcinoma
Organism
Mouse
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description


The mouse Epcam gene was replaced by human EPCAM coding sequence in B-hEPCAM MC38 cells. Human EPCAM is highly expressed on the surface of B-hEPCAM MC38 cells.

Targeting strategy

Gene targeting strategy for B-hEPCAM MC38 cells. The exogenous promoter and human EPCAM coding sequence was inserted to replace part of murine exon 4 and all of exons 5~7. The insertion disrupts the endogenous murine Epcam gene, resulting in a non-functional transcript.

Protein expression analysis


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EPCAM expression analysis in B-hEPCAM MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hEPCAM MC38 cultures were stained with species-specific anti-EPCAM antibody. Human EPCAM was detected on the surface of B-hEPCAM MC38 cells but not wild-type MC38 cells. The 3-C12 clone of B-hEPCAM MC38 cells was used for in vivo experiments.

Tumor growth curve & Body weight changes

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Subcutaneous homograft tumor growth of B-hEPCAM MC38 cells. B-hEPCAM MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 5-8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hEPCAM MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.