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B-hPD-L1 MC38
Common name
B-hPD-L1 MC38 Catalog number     310685
Aliases CD274, PDCD1L1 Disease   Colon carcinoma
Organism
Mouse 
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description 


The mouse Pdl1 gene was replaced by human PD-L1 coding sequence in B-hPD-L1 MC38 cells. Human PD-L1 is highly expressed on the surface of B-hPD-L1 MC38 cells.


Application


B-hPD-L1 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies.


Targeting strategy


The exogenous promoter and human PD-L1 coding sequence was inserted to replace part of murine exon 3. The insertion disrupts the endogenous murine Pdl1 gene, resulting in a non-functional transcript.


Protein expression analysis 


PD-L1 expression analysis in B-hPD-L1 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hPD-L1 MC38 cultures were stained with species-specific anti-PD-L1 antibody. Mouse PD-L1 was detected on the surface of wild-type MC38 cells. Human PD-L1 was detected on the surface of B-hPD-L1 MC38 cells but not wild-type MC38 cells. The D1 clone of B-hPD-L1 MC38 cells was used for in vivo experiments.


Tumor growth curve & Body weight changes




Subcutaneous homograft tumor growth of B-hPD-L1 MC38 cells. B-hPD-L1 MC38 cells (1x106, 5x105 and 2x105) were subcutaneously implanted into C57BL/6 mice (female, 6-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hPD-L1 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.