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B-hPD-L1 low B16-F10
Common name
B-hPD-L1 low B16-F10 Catalog number     310890
Aliases B7-H, B7H1, PDCD1L1, PDCD1LG1 Disease  Melanoma
Organism
Mouse
Strain  C57BL/6
Tissue types Skin Tissue  Skin

Description 


The mouse Pdl1 gene was replaced by human PD-L1 coding sequence in B-hPD-L1 low B16-F10 cells. Human PD-L1 is expressed on the surface of B-hPD-L1 low B16-F10 cells.


Application


B-hPD-L1 low B16-F10 cells have the capability to establish tumors in vivo and can be used for efficacy studies.


Targeting strategy


The exogenous promoter and human PD-L1 coding sequence was inserted to replace part of murine exon 3. The insertion disrupts the endogenous murine Pdl1 gene, resulting in a non-functional transcript.


Protein expression analysis 


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PD-L1 expression analysis in B-hPD-L1 low B16-F10 cells by flow cytometry. Single cell suspensions from wild-type B16-F10, B-hPD-L1 B16-F10 and B-hPD-L1 low B16-F10 cultures were stained with species-specific anti-PD-L1 antibody. Mouse PD-L1 was detectable in wild-type B16-F10 cells. Human PD-L1 was detectable in B-hPD-L1 low B16-F10 cells, and human PD-L1 was expressed highly on the surface of B-hPD-L1 B16-F10 cells. The 2-G02 clone of B-hPD-L1 low B16-F10 cells was used for in vivo experiments.


Tumor growth curve & Body weight changes


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Subcutaneous homograft tumor growth of B-hPD-L1 low B16-F10 cells. Wild-type B16-F10, B-hPD-L1 B16-F10 and B-hPD-L1 low B16-F10 cells (2x105) were subcutaneously implanted into C57BL/6 mice (female, 7-8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hPD-L1 low B16-F10 cells were able to establish tumors in vivo and can be used for efficacy studies.