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B-hTROP2 MC38 plus
Common name
B-hTROP2 MC38 plus Catalog number     311584
Aliases tumor associated calcium signal transducer 2, TACSTD2, EG Disease  Colon carcinoma
Organism
Mouse
Strain  C57BL/6
Tissue types Colon Tissue  Colon

Description



Expression cassettes containing the exogenous promoter and human TROP2 CDS were randomly inserted into the genome of B-hTROP2 MC38 plus cells. Human TROP2 was detected to be highly expressed on the surface of B-hTROP2 MC38 plus cells.

Application

B-hTROP2 MC38 plus cells can form tumors in vivo and can be used for efficacy studies.

Targeting strategy

Gene targeting strategy for B-hTROP2 MC38 plus cells. 
Expression cassettes containing the exogenous promoter and human TROP2 CDS were randomly inserted into the genome of MC38 cells that do not express the mouse TROP2 gene themselves.

Protein expression analysis 

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Human TROP2 expression analysis in B-hTROP2 MC38 plus cells by flow cytometry. 
Single cell suspensions of wild-type MC38 and B-hTROP2 MC38 plus cultures were stained with species-specific anti-TROP2 antibody. Human TROP2 was detected on the surface of B-hTROP2 MC38 plus cells but not wild-type MC38 cells. The 2-B10 clone of B-hTROP2 MC38 plus cells was used for in vivo tumor growth assays.

Tumor growth curve & Body weight changes



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Subcutaneous homograft tumor growth of B-hTROP2 MC38 plus cells. 
B-hTROP2 MC38 plus cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean±SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hTROP2 MC38 plus cells were able to form tumors in vivo and can be used for efficacy studies.