Strain Name |
NOD.CB17-Prkdcscid Il2rgtm1Bcgen H2-K1tm1Bcgen H2-Ab1tm1Bcgen H2-D1tm1Bcgen/Bcgen
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Common Name |
huPBMC-B-NDG MHC I/II DKO mice ad
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Background | B-NDG | Catalog number |
114316 |
Related Genes |
Prkdc: DNA-PKcs, DNAPDcs, DNAPK, DNPK1, DOXNPH, HYRC1, XRCC7, dxnph, p460, scid, slip Il2rg: CD132, [g]c, gamma(c), gc, p64 H2-K1: H-2K, H-2K(d), H2-D1, H2-K, K-f; H2-Ab1: AI845868, Abeta, H-2Ab, H2-Ab, I-Abeta, IAb, Ia-2, Ia2, Rmcs1; H2-D1: H-2D, H2-D, H2-K1
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NCBI Gene ID |
19090, 16186, 14972, 14961, 14964 |
Description
Targeting Strategy
Gene targeting strategy for B-NDG MHC I/II DKO mice ad. The exons 2-8 of mouse H2-K1 and H2-D1 gene, the exons 2-4 of mouse H2-Ab1 gene were knocked out in B-NDG MHC I/II DKO mice ad. So the transcription and translation of mouse H2-K1, H2-D1, H2-Ab1 genes will be disrupted.
A Successful Human PBMCs Engrafted Mouse Model That Can Extend the Window Period of Experiments
B-NDG MHC I/II DKO mice ad has a long life span and reduced severity of GvHD when engrafted with human PBMCs. B-NDG MHC I/II DKO mice ad were engrafted intravenously with human PBMCs (1×107) from two donors (Donor 1, Donor 2) on day 0 (n=6/8). Survival rates of the mice were analyzed with Kaplan Meier survival curves. Body weight was measured twice a week. Clinical signs of GvHD were scored once a week. Euthanasia was implemented when the body weight decreased more than 20%. Meanwhile, the GvHD score of the mouse was recorded as 10. The results showed that at 8 weeks after PBMC engraftment, the mice did not exhibit significant symptoms of GvHD and their body weight continued to increase. Values were expressed as mean ± SEM.
Reconstitution Level of Human Immune Cells by Human PBMCs Engraftment
Human PBMCs were successfully reconstituted in B-NDG MHC I/II DKO mice ad. B-NDG MHC I/II DKO mice ad were engrafted intravenously with human PBMCs (1×107) from two donors on day 0 (n=6/8). Peripheral blood was taken to analyze the reconstitution level of human immune cells. A. Frequency of reconstituted human immune cells; B. Absolute cell number of reconstituted human immune cells. The results showed that two weeks after the reconstitution of human PBMCs in B-NDG MHC I/II DKO mice ad, the frequency and absolute cell number of CD45+ cells in peripheral blood began to increase. The frequency of reconstituted human T cells exceeded 90% from three weeks and continued to rise, eventually reaching nearly 100%. Reconstituted human T cells include CD4+T cells, CD8+T cells and Tregs. A small amount of DCs can also be detected. This indicates that B-NDG MHC I/II DKO mice ad is a powerful immunodeficient mouse model for reconstitution of the human T cells using human PBMCs.